OVARIAN 3-BETA-HYDROXYSTEROID DEHYDROGENASE DELTA(5-4)-ISOMERASE OF RAINBOW-TROUT - ITS CDNA CLONING AND PROPERTIES OF THE ENZYME EXPRESSED IN A MAMMALIAN-CELL

被引：55

作者：

SAKAI, N

TANAKA, M

TAKAHASHI, M

FUKADA, S

MASON, JI

NAGAHAMA, Y

机构：

[1] NATL INST BASIC BIOL, REPROD BIOL LAB, OKAZAKI, AICHI 444, JAPAN

[2] FUKUI PREFECTURAL UNIV, DEPT MARINE BIOSCI, OBAMA, FUKUI 917, JAPAN

[3] UNIV TEXAS, SW MED CTR, CECIL H & IDA GREEN CTR REPROD BIOL SCI, DEPT BIOCHEM, DALLAS, TX 75235 USA

[4] UNIV TEXAS, SW MED CTR, CECIL H & IDA GREEN CTR REPROD BIOL SCI, DEPT OBSTET & GYNECOL, DALLAS, TX 75235 USA

来源：

FEBS LETTERS | 1994年 / 350卷 / 2-3期

关键词：

RAINBOW TROUT; 3-BETA-HSD ISOMERASE; CDNA CLONING; EXPRESSION IN COS-1 CELL;

D O I：

10.1016/0014-5793(94)00795-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone encoding 3 beta-hydroxysteroid dehydrogenase /Delta(5-4)-isomerase (3 beta-HSD) was isolated from a cDNA library of rainbow trout ovarian thecal cells. Southern hybridization analysis of trout genomic DNA with the cDNA suggested the presence of a single gene encoding 3 beta-HSD in the rainbow trout showing a total genomic size of less than 4 kilobases (kb). The cDNA hybridized to a single species of mRNA isolated from rainbow trout ovaries; the 1.4 kb transcripts were most abundant in trout ovaries during the later stages of oogenesis. The trout 3 beta-HSD expressed in non-steroidogenic monkey kidney tumor (COS-l) cells showed a unique enzymatic 3 beta-HSD activity. Dehydroepiandrosterone was a more favoral substrate of the trout 3 beta-HSD than 17 alpha-hydroxypregnenolone. Interestingly, the trout 3 beta-HSD expressed in COS-1 cells exhibited minimal ability to convert pregnenolone to progesterone.

引用

页码：309 / 313

页数：5

共 27 条

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