PROCESS OF EPITHELIAL-CELL ATTACHMENT TO GLASS SURFACES STUDIED BY REFLECTION CONTRAST MICROSCOPY

被引:15
作者
COTTLERFOX, M
SPARRING, KM
ZETTERBERG, A
FOX, CH
机构
[1] KAROLINSKA HOSP,DEPT CLIN CHEM,S-10401 STOCKHOLM,SWEDEN
[2] KAROLINSKA HOSP,INST TUMOR PATHOL,S-10401 STOCKHOLM,SWEDEN
[3] KAROLINSKA HOSP,DEPT PATHOL,S-10401 STOCKHOLM,SWEDEN
[4] NCI,BIOCHEM LAB,BETHESDA,MD 20014
关键词
D O I
10.1016/0014-4827(79)90169-1
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The process of attachment was studied in primary mouse kidney epithelial cell cultures by means of reflexion contrast microscopy, a method developed for studying the cell membrane-substrate relationship. The first in a series of events is simple adherence to the substrate, called close contact. This phenomenon is associated with the greatest extension of lamellar cytoplasm and the fewest number of cell nuclei/unit area. The nuclei of such cells are in close contact with the bottom portion of the cell membrane. Approx. 24 h after planting, as the cultures become more crowded, cells develop a different kind of attachment to the substrate-focal contacts-that are correlated with a decrease in lamellar cytoplasm. Cells detached from the substrate after close contact formation readily reattach, while cells detached after formation of focal contacts do not reattach. After incubation for periods greater than 5 days, the dense cultures degenerate and cells lose their attachment to the glass surface. © 1979.
引用
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页码:414 / 418
页数:5
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