PRIMARY STRUCTURE OF THE S-PEPLOMER GENE OF BOVINE CORONAVIRUS AND SURFACE EXPRESSION IN INSECT CELLS

被引：29

作者：

PARKER, MD ^{[1
]}

YOO, D ^{[1
]}

COX, GJ ^{[1
]}

BABIUK, LA ^{[1
]}

机构：

[1] UNIV SASKATCHEWAN,DEPT VET MED,SASKATOON S7N 0W0,SASKATCHEWAN,CANADA

来源：

JOURNAL OF GENERAL VIROLOGY | 1990年 / 71卷

关键词：

D O I：

10.1099/0022-1317-71-2-263

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nucleotide sequence of the S peplomer gene of bovine coronavirus (BCV) has been determined. A single open reading frame of 4089 nucleotides encodes a polypeptide of 150K with 20 potential sites for addition of N-linked oligosaccharides. Expression of the cloned BCV S gene by a recombinant Autographa californica nuclear polyhedrosis virus resulted in production of a 180K glycosylated polypeptide which was transported to the surface of the cell. Comparison of the BCV S gene with the analogous genes of murine hepatitis viruses shows that the BCV S polypeptide contains a unique domain of 138 amino acids not present in murine hepatitis virus strain JHM, but which has a partially homologous counterpart in strain A59. This domain accounts for most of the differences in size of the S gene products of these coronaviruses.

引用

页码：263 / 270

页数：8

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