CELL-CULTURE OF THE INTERVERTEBRAL-DISK OF RATS - FACTORS INFLUENCING CULTURE, PROTEOGLYCAN, COLLAGEN, AND DEOXYRIBONUCLEIC-ACID SYNTHESIS

被引：54

作者：

ICHIMURA, K ^{[1
]}

TSUJI, H ^{[1
]}

MATSUI, H ^{[1
]}

MAKIYAMA, N ^{[1
]}

机构：

[1] TOYAMA MED & PHARMACEUT UNIV,FAC MED,DEPT ORTHOPAED SURG,2630 SUGITANI,SUGITANI,TOYAMA 93001,JAPAN

来源：

JOURNAL OF SPINAL DISORDERS | 1991年 / 4卷 / 04期

关键词：

INTERVERTEBRAL DISK; CELL CULTURE; PROTEOGLYCAN; DEOXYRIBONUCLEIC ACID; COLLAGEN;

D O I：

10.1097/00002517-199112000-00004

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

To establish cell culture of the nucleus pulposus and anulus fibrosus of rat intervertebral disc, the effects of culture conditions on the growth of cells and the synthesis of DNA, proteoglycan, and collagen were studied. For cell culture of the nucleus pulposus, the use of 3-week-old rats and a medium adjusted to pH 7.0 was optimal. There was almost no difference in growth between cells in Ham's F12 medium and those in Dulbecco's Modified Eagle Medium. In cells isolated from the anulus fibrosus, a medium adjusted to pH 7.0-7.6 was preferable, but irrespective of rat age. Culture cells of the nucleus pulposus were composed of large cells with vacuoles and small polygonal cells. These cells had a slight growth activity and a fair capability of proteoglycan and collagen synthesis. Culture cells of the anulus fibrosus were composed of polygonal and spindle-shape cells, and the growth was more vigorous with the potentials for proteoglycan and collagen synthesis than the nucleus cells.

引用

页码：428 / 436

页数：9

共 24 条

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