INVITRO FERTILIZATION IN CASES WITH SEVERE SPERM DEFECT - USE OF A SWIM-ACROSS TECHNIQUE AND MEDIUM SUPPLEMENTED WITH FOLLICULAR-FLUID

被引：11

作者：

GIORGETTI, C

HANS, E

SPACH, JL

AUQUIER, P

ROULIER, R

机构：

[1] Institut de Médecine de la Reproduction, 13008 Marseille

[2] Laboratoire de Santé Publique, Faculté de Médecine, 13385 Marseille

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 08期

关键词：

FOLLICULAR FLUID; SEVERE SPERM DEFECT; SWIM-ACROSS;

D O I：

10.1093/oxfordjournals.humrep.a137805

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

The purpose of this study was to evaluate a new method of in-vitro fertilization (IVF) in patients with severe sperm defects. Unlike the conventional swim-up method, spermatozoa and oocytes are placed in opposite corners of the bottom of the incubation dish so that sperm swimming is horizontal instead of vertical. Another difference between the swim-across and swim-up techniques is that the incubation medium is supplemented with 20% follicular fluid. After a randomized series (protocol I) of 15 IVF attempts had demonstrated that swim-across was more effective than swim-up in terms of fertilization and cleavage, we began a second series (protocol II) using only swim-across. A total of 124 couples with motile sperm counts <1 x 10(6) spermatozoa/ml of semen were included in protocol II. Clinical parameters (age, tubal damage) and number of recovered oocytes were recorded and compared in patients who did (group A: n = 94) and did not (group B: n = 74) achieve fertilization. In group A the fertilization rate was 36.7% and, out of the 94 transfers that were made, there were 21 clinical pregnancies and 12 full-term pregnancies with 16 live births. The number of oocytes collected (12 versus 7.7, P < 0.001) and the incidence of tubal damage (50% versus 24.3%, P < 0.001) was significantly higher in group A than in group B. Using logistic regression analysis, we showed a significant correlation between fertilization and progressive motility, percentage normal spermatozoa, number of recovered oocytes and tubal damage. The efficacy of this method may be due to two factors: more efficient utilization of motile spermatozoa, and follicular fluid supplementation. These results demonstrate that fertilization and full-term pregnancy can be achieved with extremely low motile sperm counts.

引用

页码：1121 / 1125

页数：5

共 31 条

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