IMMOBILIZATION OF IMMUNOGLOBULIN ON A QUARTZ SURFACE BY BRCN WITHOUT LOSS OF ANTIGEN-BINDING CAPABILITY

被引：10

作者：

KRAPIVINSKAYA, LD ^{[1
]}

KRAPIVINSKY, GB ^{[1
]}

RATNER, VL ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI,INST CELL BIOPHYS,MOSCOW 142292,USSR

来源：

BIOSENSORS & BIOELECTRONICS | 1992年 / 7卷 / 07期

关键词：

IMMOBILIZATION; IMMUNOGLOBULIN; BRCN ACTIVATION; QUARTZ;

D O I：

10.1016/0956-5663(92)80008-Y

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

BrCN-activated quartz was used for the immobilization of monoclonal immunoglobulins. BrCN-activated quartz keeps its ability to quantitatively bind immunoglobulins for at least 8 h. The surface density of the immobilized immunoglobulins was 100 ng cm-2 and they were found to retain their ability to bind antigen in a molar ratio of 1:2 for not less than three months.

引用

页码：509 / 513

页数：5

共 18 条

[1]

BEKA S, 1988, METHOD ENZYMOL, V135, P160

[2]

CATHOU RE, 1978, IMMUNOGLOBULINS, P58

[3]

Fields R, 1972, Methods Enzymol, V25, P464, DOI 10.1016/S0076-6879(72)25042-X

[4] THIOPHILIC ADSORPTION OF IMMUNOGLOBULINS - ANALYSIS OF CONDITIONS OPTIMAL FOR SELECTIVE IMMOBILIZATION AND PURIFICATION [J].

HUTCHENS, TW ;

PORATH, J .

ANALYTICAL BIOCHEMISTRY, 1986, 159 (01) :217-226

[5] GLUTARALDEHYDE FIXATION OF THE PRIMARY ANTIBODY-ANTIGEN COMPLEX ON NITROCELLULOSE PAPER INCREASES THE OVERALL SENSITIVITY OF IMMUNOBLOT ASSAY [J].

IKEGAKI, N ;

KENNETT, RH .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 124 (02) :205-210

[6]

JASIM MA, 1987, J CHEM TECHNOL BIOT, V40, P251

[7]

JONSSON U, 1988, METHOD ENZYMOL, V137, P381

[8] THE USE OF CYANOGEN-BROMIDE AND OTHER NOVEL CYANYLATING AGENTS FOR THE ACTIVATION OF POLYSACCHARIDE RESINS [J].

KOHN, J ;

WILCHEK, M .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1984, 9 (03) :285-305

[9] NEW IMMUNOASSAY BASED ON FLUORESCENCE EXCITATION BY INTERNAL-REFLECTION SPECTROSCOPY [J].

KRONICK, MN ;

LITTLE, WA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1975, 8 (03) :235-240

[10]

Lynn M., 1975, IMMOBILIZED ENZYMES, P1

← 1 2 →