COTRANSLATIONAL HEME-BINDING TO NASCENT GLOBIN CHAINS

被引：47

作者：

KOMAR, AA ^{[1
]}

KOMMER, A ^{[1
]}

KRASHENINNIKOV, IA ^{[1
]}

SPIRIN, AS ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI, INST PROT RES, PUSHCHINO 142292, RUSSIA

来源：

FEBS LETTERS | 1993年 / 326卷 / 1-3期

关键词：

HEMOGLOBIN; HEME; ATTACHMENT; NASCENT PEPTIDE; COTRANSLATIONAL FOLDING;

D O I：

10.1016/0014-5793(93)81803-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Globin synthesis in cell-free extracts of rabbit reticulocytes was carried out in the presence of H-3-labeled hemin. Sucrose gradient centrifugation analysis revealed [H-3]hemin in the polyribosome fraction. The addition of puromycin resulted in the release of both [H-3]hemin- and [C-14]leucine-labeled polypeptide from the polyribosomes. The data suggest cotranslational folding of the globin molecule on the ribosome and cotranslational heme binding to the nascent globin chain.

引用

页码：261 / 263

页数：3

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