INTRACELLULAR-LOCALIZATION OF SUCROSE-PHOSPHATE PHOSPHATASE IN PHOTOSYNTHETIC CELLS OF LETTUCE (LACTUCA-SATIVA)

被引：8

作者：

ECHEVERRIA, E ^{[1
]}

SALERNO, G ^{[1
]}

机构：

[1] UNIV FLORIDA,CTR AGR RES & EDUC,LAKE ALFRED,FL 33850

来源：

PHYSIOLOGIA PLANTARUM | 1993年 / 88卷 / 03期

关键词：

SUCROSE SYNTHESIS; VACUOLE;

D O I：

10.1111/j.1399-3054.1993.tb01356.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The intracellular localization of sucrose-phosphate phosphatase (SPP) in photosynthetic cells of lettuce (Lactuca sativa) was investigated by using protoplasts isolated directly from leaf tissue. No SPP activity was detected in either vacuolar, chloroplast or mitochondrial preparations. The recovered activity of SPP was found in the cytosolic fractions at proportions parallel with those of the cytoplasmic marker alcohol dehydrogenase. Maximal activity was measured between pH 6.0 and 7.0. The data demonstrate a cytosolic location for SPP. Neither sucrose nor any other saccharide tested at 100 mM was a strong inhibitor of the enzyme, which was inactive in the presence of 35 mM ethylenediaminetetraacetic acid.

引用

页码：434 / 438

页数：5

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