LINKAGE ANALYSIS OF ER-1, A RECESSIVE PISUM-SATIVUM GENE FOR RESISTANCE TO POWDERY MILDEW FUNGUS (ERYSIPHE-PISI DC)

被引：89

作者：

TIMMERMAN, GM ^{[1
]}

FREW, TJ ^{[1
]}

WEEDEN, NF ^{[1
]}

MILLER, AL ^{[1
]}

GOULDEN, DS ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT HORT SCI,GENEVA,NY 14456

来源：

THEORETICAL AND APPLIED GENETICS | 1994年 / 88卷 / 08期

关键词：

PEA; POWDERY MILDEW; GENOME MAPPING; MOLECULAR MARKERS; BULKED SEGREGANT ANALYSIS;

D O I：

10.1007/BF00220815

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F-2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR).

引用

页码：1050 / 1055

页数：6

共 30 条

[1] AMINE-CITRATE BUFFERS FOR PH CONTROL IN STARCH-GEL ELECTROPHORESIS [J].