STRONGLY BASIC ANION EXCHANGE RESINS PREPARED FROM DIFFERENT POLYMER MATRICES AND THEIR APPLICATION TO SEPARATION OF PROTEINS IN HIGH PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY

被引：6

作者：

Etoh, Takashi ^{[1
]}

Miyazaki, Mika ^{[1
]}

Iwatake, Miyuki ^{[1
]}

Harada, Kumiko ^{[1
]}

Nakayama, Morio ^{[1
]}

Sugii, Atsushi ^{[1
]}

机构：

[1] Kumamoto Univ, Fac Pharmaceut Sci, Kumamoto 862, Japan

来源：

ANALYTICAL SCIENCES | 1991年 / 7卷

关键词：

protein; ion-exchange chromatography; HPLC;

D O I：

10.2116/analsci.7.Supple_187

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Quaternized dimethylaminomethylstyrene-ethylene glycol dimethacrylate(EGDMA) (PDAMS-Q) and chloromethylstyrene-EGDMA(PCMS-Q) were prepared as column packings having the same functional groups for HPIEC of proteins. These resins prepared from different polymer matrices varied from each other in the retention behavior of proteins. PDAMS-Q column was suitable for analysis of acidic proteins, but PCMS-Q eluted all proteins at void volume.

引用

页码：187 / 190

页数：4

共 5 条

[1] HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY OF PROTEINS
KADOYA, T
ISOBE, T
AMANO, Y
KATO, Y
NAKAMURA, K
OKUYAMA, T
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1985, 8 (04): : 635 - 650
[2] NEW HIGH-PERFORMANCE CATION EXCHANGER FOR THE SEPARATION OF PROTEINS
KATO, Y
NAKAMURA, K
HASHIMOTO, T
[J]. JOURNAL OF CHROMATOGRAPHY, 1984, 294 (JUN): : 207 - 212
[3] NEW ION-EXCHANGER FOR THE SEPARATION OF PROTEINS AND NUCLEIC-ACIDS
KATO, Y
NAKAMURA, K
HASHIMOTO, T
[J]. JOURNAL OF CHROMATOGRAPHY, 1983, 266 (AUG): : 385 - 394
[4] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF SERUM ALBUMINS ON AN N-METHYLPYRIDINIUM POLYMER-BASED COLUMN
NISHIMURA, K
HARADA, K
MASUDA, S
SUGII, A
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 525 (01): : 176 - 182
[5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PROTEINS ON N-METHYLPYRIDINIUM POLYMER COLUMNS
SUGII, A
HARADA, K
NISHIMURA, K
HANAOKA, R
MASUDA, S
[J]. JOURNAL OF CHROMATOGRAPHY, 1989, 472 (02): : 357 - 364

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