SYNTHESIS OF ACTIN BY CULTURED GUINEA-PIG MEGAKARYOCYTES - COMPLEX-FORMATION WITH FIBRIN

被引：24

作者：

NACHMAN, R ^{[1
]}

LEVINE, R ^{[1
]}

JAFFE, E ^{[1
]}

机构：

[1] VET ADM HOSP, WASHINGTON, DC 20422 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 543卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0304-4165(78)90457-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Guine pig megakaryocytes were isolated from femoral marrow and cultured in the presence of radioactive amino acids. Radioactivity was incorporated into several proteins including a 42 000 dalton polypeptide ideitified as actin by DNAase agarose affinity chromatography. Quantitative immunonoelectrophoresis of megakaryocytes extract revealed that 3.0% of the total solubilized cellular protein was fibrinogen. Immunoabsorption studies using anti guinea pig fibrinogen beads failed to revealed the presence of newly synthesized radioactive fibrinogen in the cellular extract, however, radioactive actin was detected in the eluates obtained from the immune beads. When guinea pig fibrinogen was clotted with trombin in the presence of radioactive megakaryocyte extract, a complex fromed between a high molecular weight species of fibrin and actin. No actin · fibrinogen complex was detected. The results suggest that actin synthesized by megakartocytes complexes with fibrin formed from a relatively large pool of non-radioactive intracellular fibrinogen. © 1978.

引用

页码：91 / 105

页数：15

共 32 条

[1] COMPLEX FORMATION WITH HEAVY MEROMYOSIN OF ISOLATED ACTIN-LIKE COMPONENT OF THROMBOSTHENIN, CONTRACTILE PROTEIN FROM BLOOD-PLATELETS [J].