LONG-RANGE PHYSICAL MAPPING AROUND THE HUMAN STEROID SULFATASE LOCUS

被引：27

作者：

ROSS, MT

BALLABIO, A

CRAIG, IW

机构：

[1] BAYLOR UNIV,HOWARD HUGHES MED INST,HOUSTON,TX 77030

[2] BAYLOR UNIV,INST MOLEC GENET,HOUSTON,TX 77030

来源：

GENOMICS | 1990年 / 6卷 / 03期

关键词：

D O I：

10.1016/0888-7543(90)90482-A

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The region of the human X chromosome containing the steroid sulfatase locus was analyzed by pulsed-field gel electrophoresis. Restriction site maps were generated for the X chromosome in the blood of a normal male individual and that in the mouse-human hybrid cell line ThyB-X; these maps extend over approximately 4.3 Mb of DNA of the former, and 3.2 Mb of the latter. Physical linkage was defined between the STS locus and sequences detected by the probes GMGX9 (DXS237), GMGXY19 (DYS74), CRI-S232 (DXS278), and dic56 (DXS143), and the order telomere-(STS, DYS74)-DXS237-DXS278-DXS143-centromere was deduced. The pulsed-field maps were used to demonstrate a deletion of 180 kb of DNA from the X chromosome of an individual with X-linked ichthyosis. Also, possible locations for the Kallmann syndrome gene were revealed, and the distance between the steroid sulfatase locus and the pseudoautosomal region was estimated to be at least 4 Mb. © 1990.

引用

页码：528 / 539

页数：12

共 40 条

[1] MAPPING THE TESTIS DETERMINANTS BY AN ANALYSIS OF Y-SPECIFIC SEQUENCES IN MALES WITH APPARENT XX AND XO KARYOTYPES AND FEMALES WITH XY KARYOTYPES [J].