FREEZING RABBIT SEMEN BY THE USE OF BF5 DILUENT

被引：16

作者：

BAMBA, K ^{[1
]}

ADAMS, CE ^{[1
]}

机构：

[1] AFRC, INST ANIM PHYSIOL, ANIM RES STN, CAMBRIDGE CB3 0JQ, ENGLAND

来源：

LABORATORY ANIMALS | 1990年 / 24卷 / 02期

关键词：

Cryoprotective Agents; Freezing Spermatozoa; Rabbit;

D O I：

10.1258/002367790780890149

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Three experiments were carried out to find the optimal concentration of DMSO and glycerol in BFS diluent for freezing rabbit spermatozoa. Semen was diluted 1:1 with diluent A (BF5 + DMSO) at 25°C and diluted further 1:1 with diluent B (diluent A + glycerol) after cooling down to 5°C. Diluted semen was frozen immediately and stored in liquid nitrogen. Maximum percentages for motility and normal acrosomes were obtained in the presence of 12% DMSO (as expressed in diluent A) and 3% glycerol (final concentration) after thawing. © 1990, Royal Society of Medicine Press. All rights reserved.

引用

页码：172 / 175

页数：4

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