H-1-NMR DETERMINATION OF THE IONIZATION-CONSTANT OF A CARBOXYLIC-ACID GROUP OF A HEME PROTEIN IN MIXED AQUEOUS-ORGANIC SOLVENTS USING THE SUPER WEFT SEQUENCE

被引：6

作者：

RIGBY, SEJ ^{[1
]}

BURCH, AM ^{[1
]}

MOORE, GR ^{[1
]}

机构：

[1] UNIV E ANGLIA,SCH CHEM SCI,CTR METALLOPROTEIN SPECTROSCOPY & BIOL,NORWICH NR4 7TJ,NORFOLK,ENGLAND

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 1991年 / 29卷 / 10期

关键词：

ELECTROSTATICS; CYTOCHROME; SUPERWEFT; DIELECTRIC CONSTANT; NOE;

D O I：

10.1002/mrc.1260291011

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

H-1 NMR spectra of ferricytochrome c-551 were obtained in a variety of miscible aqueous buffer-organic solvent mixtures. The pK(a) of a haem carboxyl group within this protein was measured in several such solvent systems. This work was facilitated by the use of the Super water eliminated Fourier transform (SuperWEFT) technique and this was extended to the suppression of multiple solvent resonances. Nuclear Overhauser enhancement difference experiments were combined with SuperWEFT, enabling structural data to be obtained in complex solvent mixtures. The data arising from this study are relevant to the evaluation and simulation of electrostatic effects in proteins.

引用

页码：1036 / 1039

页数：4

共 23 条

[1] SPIN DIFFUSION AND THE DYNAMIC STRUCTURE OF A PROTEIN - STREPTOMYCES SUBTILISIN INHIBITOR [J].