IDENTIFICATION OF 3',5'-CYCLIC-AMP IN AXENIC CULTURES OF LEMNA-PAUCICOSTATA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：14

作者：

GANGWANI, L ^{[1
]}

TAMOT, BK ^{[1
]}

KHURANA, JP ^{[1
]}

MAHESHWARI, SC ^{[1
]}

机构：

[1] TRIBHUVAN UNIV,CENT DEPT BOT,KATMANDU,NEPAL

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)91007-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleotides were purified from the extract of aseptically grown Lemna paucicostata 6746 by low-pressure column chromatography and thin-layer chromatography. In this partially purified sample, a compound has been identified, using a highly sensitive HPLC-fluorometric detection method, with chromatographic properties and substrate specificity for cyclic nucleotide phosphodiesterase similar to authentic 3′,5′-cyclic AMP. The level of cAMP has been estimated to be 70-80 pmol/g fresh wt. © 1991.

引用

页码：1113 / 1119

页数：7

共 23 条

[1] THE MOLECULAR-BASIS OF COMMUNICATION WITHIN THE CELL
BERRIDGE, MJ
[J]. SCIENTIFIC AMERICAN, 1985, 253 (04) : 142 - &
[2] Growth factor requirements of four species of isolated roots
Bonner, J
Devirian, PS
[J]. AMERICAN JOURNAL OF BOTANY, 1939, 26 (08) : 661 - 665
[3] AN ASSAY-METHOD FOR CYCLIC-AMP USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PRETREATMENT BY ALKALINE-PHOSPHATASE
FURUYA, E
KUROSAWA, K
HAGIHARA, B
[J]. ANALYTICAL BIOCHEMISTRY, 1985, 145 (01) : 144 - 147
[4] PARTIAL CHARACTERIZATION AND LIGHT-INDUCED REGULATION OF GTP-BINDING PROTEINS IN LEMNA-PAUCICOSTATA
HASUNUMA, K
FURUKAWA, K
FUNADERA, K
KUBOTA, M
WATANABE, M
[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1987, 46 (04) : 531 - 535
[5] GTP-BINDING PROTEIN(S) IN GREEN PLANT, LEMNA-PAUCICOSTATA
HASUNUMA, K
FUNADERA, K
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 143 (03) : 908 - 912
[6] HASUNUMA K, 1988, PHOTOCHEM PHOTOBIOL, V48, P89
[7] REVERSED PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATIONS OF NUCLEOTIDES IN PRESENCE OF SOLVOPHOBIC IONS
HOFFMAN, NE
LIAO, JC
[J]. ANALYTICAL CHEMISTRY, 1977, 49 (14) : 2231 - 2234
[8] EPINEPHRINE, PROPRANOLOL, AND THE SUCROSE-AMMONIUM INHIBITION OF FLOWERING IN LEMNA-PAUCICOSTATA 6746
IVES, JM
POSNER, HB
[J]. PLANT PHYSIOLOGY, 1982, 70 (01) : 311 - 312
[9] EFFECTS OF CYCLIC-AMP ON THE ACTIVITY OF SOLUBLE-PROTEIN KINASES IN LEMNA-PAUCICOSTATA
KATO, R
UNO, I
ISHIKAWA, T
FUJII, T
[J]. PLANT AND CELL PHYSIOLOGY, 1983, 24 (05) : 841 - 848
[10] KATO R, 1988, PLANT CELL PHYSIOL, V29, P85

← 1 2 3 →