DETECTION OF CHIMERISM AFTER BONE-MARROW TRANSPLANTATION USING THE DOUBLE AMPLIFICATION REFRACTORY MUTATION SYSTEM

被引：12

作者：

LO, YMD

ROUX, E

JEANNET, M

CHAPUIS, B

FLEMING, KA

WAINSCOAT, JS

机构：

[1] JOHN RADCLIFFE HOSP,DEPT HAEMATOL,LRF MOLEC & CYTOGENET HAEMATOL UNIT,OXFORD OX3 9DU,ENGLAND

[2] HOP CANTONAL UNIV,GENEVA,SWITZERLAND

[3] JOHN RADCLIFFE HOSP,NUFFIELD DEPT PATHOL & BACTERIOL,OXFORD OX3 9DU,ENGLAND

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 1993年 / 85卷 / 01期

关键词：

D O I：

10.1111/j.1365-2141.1993.tb08679.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have developed a highly sensitive double amplification refractory mutation system (double ARMS) for the detection of extremely low levels of mixed chimaerism after bone marrow transplantation. The system we chose for double ARMS analysis is a highly polymorphic region 5' to the human delta-globin gene. Double ARMS analysis was found to correlate well with an established minisatellite system and was shown to demonstrate a very low level of mixed chimaerism in cases undetectable by the latter method.

引用

页码：223 / 226

页数：4

共 9 条

[1]

LAWLER M, 1991, BLOOD, V77, P2504

[2] CHIMERISM FOLLOWING ALLOGENEIC BONE-MARROW TRANSPLANTATION - DETECTION OF RESIDUAL HOST-CELLS USING THE POLYMERASE CHAIN-REACTION [J].