HYALURONATE SYNTHASE - CLONING AND SEQUENCING OF THE GENE FROM STREPTOCOCCUS SP

被引：37

作者：

LANSING, M

LELLIG, S

MAUSOLF, A

MARTINI, I

CRESCENZI, F

OREGAN, M

PREHM, P

机构：

[1] INST PHYSIOL CHEM & PATHOBIOCHEM, WALDEYERSTR 15, W-4400 MUNSTER, GERMANY

[2] FIDIA RES LABS, DIV ADV TECHNOL, I-35031 ABANO TERME, ITALY

[3] MAX PLANCK INST BIOCHEM, W-8033 MARTINSRIED, GERMANY

来源：

BIOCHEMICAL JOURNAL | 1993年 / 289卷

关键词：

D O I：

10.1042/bj2890179

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete nucleotide sequence of hyaluronate synthase from Streptococcus sp. and its flanking regions is presented. The gene locus was designated has. Southern-blotting results suggested that the gene was conserved in hyaluronate-producing streptococci. A putative translation-initiation codon was identified and the open reading frame consists of 1566 bp, specifying a protein of 56 kDa. Sequences resembling the promoter and ribosome-binding site of Gram-positive organisms are found upstream of the synthase. The predicted amino-acid sequence reveals the presence of a 35-residue signal peptide. The sequence has some similarity to bacterial peptide-binding proteins.

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页码：179 / 184

页数：6

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