ANGIOTENSIN AND BRADYKININ INTERACTIONS WITH PHOSPHOLIPIDS

被引:9
作者
ELLIOTT, ME
GOODFRIEND, TL
机构
[1] UNIV WISCONSIN, SCH MED,DEPT INTERNAL MED, MADISON, WI 53705 USA
[2] UNIV WISCONSIN, SCH MED,DEPT PHARMACOL, MADISON, WI 53705 USA
关键词
D O I
10.1016/0304-4165(79)90105-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Reversible interactions were demonstrated between some phospholipids and some polypeptides related to angiotensin and bradykinin. The extent of the interaction was dependent on the structures of the lipid and peptide. The naturally occurring compounds that interacted most avidly were cardiolipin and (des-Asp1)-angiotensins. The apparent dissociation constant of this complex in chloroform was 10-5 M. The complex contained more than one cardiolipin molecule/molecule of peptide. Kinins interacted most strongly with lecithin. The phospholipids altered the chromatographic behavior of radioiodinated derivatives of the polypeptides, and solubilized radioactive and unlabeled polypeptides in chloroform. In aqueous media, cardiolipin suspensions preferentially bound (des-Asp1)-angiotensin II, and inhibited its binding by antibody. The interactions were sensitive to pH and cations in the aqueous phase, and were reversed by some reagents added to the organic phase. These interactions have direct implications for binding reactions of peptides in vitro, and may bear upon the actions of the hormones in vivo. © 1979.
引用
收藏
页码:357 / 373
页数:17
相关论文
共 33 条
[1]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[2]   INTERACTION OF VIBRIO-CHOLERAE ENTEROTOXIN WITH CELL-MEMBRANES [J].
CUATRECASAS, P .
BIOCHEMISTRY, 1973, 12 (18) :3547-3558
[3]   SYNTHESIS OF PROSTAGLANDINS BY RAT RENAL PAPILLA INVITRO - MECHANISM OF STIMULATION BY ANGIOTENSIN-II [J].
DANON, A ;
CHANG, LCT ;
SWEETMAN, BJ ;
NIES, AS ;
OATES, JA .
BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 388 (01) :71-83
[4]   CONFORMATIONAL-CHANGES OF PROTECTED GLUCAGON FRAGMENTS BOUND TO LYSOLECITHIN [J].
EDELHOCH, H ;
SCHNEIDER, AB .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 157 (02) :470-475
[5]  
FREEDLENDER AE, 1977, FED PROC, V36, P481
[6]  
FREEDLENDER AE, 1979, METHOD HORM RADIOIMM, P889
[7]  
FUXE K, 1977, CENTRAL ACTIONS ANGI, P515
[8]   RENIN, ANGIOTENSIN AND HYPERTENSIVE VASCULAR DAMAGE - REVIEW [J].
GIESE, J .
AMERICAN JOURNAL OF MEDICINE, 1973, 55 (03) :315-332
[9]  
GOODFRIE.TL, 1974, FED PROC, V33, P547
[10]   ANTIBODIES TO BRADYKININ + ANGIOTENSIN - USE OF CARBODIIMIDES IN IMMUNOLOGY [J].
GOODFRIEND, TL ;
LEVINE, L ;
FASMAN, GD .
SCIENCE, 1964, 144 (362) :1344-&