QUANTITATIVE-ANALYSIS OF EXOCYTOSIS DIRECTLY VISUALIZED IN LIVING CHROMAFFIN CELLS

被引：61

作者：

TERAKAWA, S ^{[1
]}

FAN, JH ^{[1
]}

KUMAKURA, K ^{[1
]}

OHARAIMAIZUMI, M ^{[1
]}

机构：

[1] SOPHIA UNIV,INST LIFE SCI,TOKYO 102,JAPAN

来源：

NEUROSCIENCE LETTERS | 1991年 / 123卷 / 01期

基金：

日本学术振兴会;

关键词：

EXOCYTOSIS; CHROMAFFIN CELL; CHOLINERGIC RECEPTOR; SECRETION; ADRENAL MEDULLA; SECRETORY GRANULE; DESENSITIZATION; VIDEO-MICROSCOPY;

D O I：

10.1016/0304-3940(91)90163-N

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Chromaffin cells isolated from the bovine adrenal medulla were observed under a Nomarski microscope through a CCD camera and an image processor. Exocytotic events of individual granules including fusion, extrusion, swelling, omega-figure formation, and membrane retrieval were visualized in individual cells stimulated by acetylcholine or K-rich solution. Initial steps were quicker than 16 ms, and the membrane retrieval was slower than 1-60 s. These findings provided a light microscopic proof for the exocytosis hypothesis as well as a basis for quantification of hormonal release. The technique was used to demonstrate significant secretion induced by a muscarinic agonist.

引用

页码：82 / 86

页数：5

共 18 条

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