MAGNETIC BEAD PURIFICATION OF M13-DNA SEQUENCING TEMPLATES

被引：18

作者：

ALDERTON, RP

ECCLESTON, LM

HOWE, RP

READ, CA

REEVE, MA

BECK, S

机构：

[1] IMPERIAL CANC RES FUND,44 LINCOLNS INN FIELDS,LONDON WC2A 3PX,ENGLAND

[2] AMERSHAM INT PLC,AMERSHAM HP7 9LL,ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 201卷 / 01期

关键词：

D O I：

10.1016/0003-2697(92)90190-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the preparation of multiple M13 DNA sequencing templates is described. No phenol extraction is required and the procedure can be carried out in Eppendorf tubes or 96-well microtiter plates. Starting with a phage supernatant, the entire procedure is carried out in the same reaction vessel and all separation steps are based on a novel application of magnetic bead separation. The design of a 96-well magnetic separator is presented and the application of the method for large-scale sequencing is discussed. © 1992.

引用

页码：166 / 169

页数：4

共 9 条

[1]

Bankier A T, 1991, DNA Seq, V2, P1, DOI 10.3109/10425179109008433

[2]

BANKIER AT, 1987, METHOD ENZYMOL, V155, P51

[3] RAPID PREPARATION OF BACTERIOPHAGE DNA FOR SEQUENCE-ANALYSIS IN SETS OF 96 CLONES, USING FILTRATION [J].