NYLON POLYETHYLENEIMINE MICROCAPSULES FOR IMMOBILIZING MULTI-ENZYMES WITH SOLUBLE DEXTRAN-NAD+ FOR CONTINUOUS RECYCLING OF MICROENCAPSULATED DEXTRAN-NAD+

被引:26
作者
GRUNWALD, J
CHANG, TMS
机构
[1] Department of Physiology, McGill University Montreal
关键词
D O I
10.1016/0006-291X(78)91572-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Semipermeable nylon polyethyleneimine microcapsules were prepared to contain a multi-enzyme system of yeast alcohol dehydrogenase (EC 1.1.1.1) and malic dehydrogenase (EC 1.1.1.37) together with a soluble immobilized coenzyme, dextran-NAD+. In the presence of the substrates ethanol and oxaloacetic acid dextran-NAD+ was successfully recycled within the microcapsules by the sequential reaction of the included enzymes. Recycling rates of 72 cycles per hour were achieved. The microcapsules were also used in a continuous flow shunt where the rate of recycling of dextran-NAD+ reaches a peak within 45 min. and remains at this maximal level throughout 4 hours of continuous reaction. The microcapsules have shown good storage stability, 50% of the activity was retained after 18 days of storage. © 1978.
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页码:565 / 570
页数:6
相关论文
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