INCORPORATION OF RADIOACTIVITY FROM 14C-NITROGLYCERIN INTO RAT LIVER GLYCOGEN LIPID PROTEIN RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID

Rats were given a single oral dose of 14C-nitroglycerin and their livers were excised after 1 hr. Fractionation of the livers showed the lipid, glycogen, protein, RNA and DNA to be radioactive. In order to be confident that the nucleic acids were actually labeled, pure RNA was isolated and hydrolyzed to its component nucleotides. The nucleotides, resolved chromatographically, were found to be labeled, indicating that radioactivity from 14C-nitroglycerin was indeed incorporated into RNA (and therefore, presumably, into DNA as well). The single treatment with nitroglycerin did not affect the glycogen, protein, lipid, RNA or DNA content of the liver. © 1969.