DETERMINATION OF URINARY AMMONIA BY OSMOMETRY

被引：8

作者：

OH, MS

PHELPS, KR

LIEBERMAN, RL

CARROLL, HJ

机构：

[1] Department of Medicine, State University of New York, Downstate Medical Center, Brooklyn

来源：

ANALYTICAL CHEMISTRY | 1979年 / 51卷 / 13期

关键词：

D O I：

10.1021/ac50049a043

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Osmometry replaces the cumbersome titration technique in this new, accurate, simple method for measuring urinary ammonia. Ammonia liberated from urine by addition of saturated K2CO3 is trapped as (NH4)2SO4 in 0.3 N H2SO4 through an aeration train. One milliliter of 0.4 N NaOH is added to 1 mL of the trapping solution and the osmolality of the mixture is measured. Sufficient NaOH is present in the mixture to titrate completely H2SO4 and (NH4)2SO4 to Na2SO4 and NH3. Since NH3 remains in solution unless it is agitated vigorously, the osmolality of this solution is greater, by the quantity of ammonia trapped, than the osmolality of a blank consisting of a mixture of the H2SO4 and NaOH. © 1979, American Chemical Society. All rights reserved.

引用

页码：2247 / 2228

页数：20

共 6 条

[1] Microdiffusion methods. Ammonia and urea using buffered absorbents (revised methods for ranges greater than 10 mu g. N) [J].