LIQUID-CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF DINICONAZOLE USING A BETA-CYCLODEXTRIN-BONDED COLUMN

Enantiomers of the fungicide diniconazole were separated by reversed-phase high-performance liquid chromatography on a commercially available beta-cyclodextrin (beta-CD)-bonded column. The effects of organic modifier in the mobile phase, mobile phase pH and column temperature on the retention and resolution of the enantiomers were studied and optimum conditions were established. The retention behaviour of some structurally related compounds of diniconazole under the optimum conditions were examined to clarify the separation mechanism on the beta-CD-bonded column. Optical separation of diniconazole using beta-CD as a mobile phase modifier on an octadecylsilanized (ODS) silica gel column was also investigated for comparison with separation by the above method.