IMMATURE SURFACE IG+ B-CELLS CAN CONTINUE TO REARRANGE KAPPA-L-CHAIN AND LAMBDA-L-CHAIN GENE LOCI

被引:121
作者
ROLINK, A [1 ]
GRAWUNDER, U [1 ]
HAASNER, D [1 ]
STRASSER, A [1 ]
MELCHERS, F [1 ]
机构
[1] ROYAL MELBOURNE HOSP,WALTER & ELIZA HALL INST MED RES,PARKVILLE,VIC 3050,AUSTRALIA
关键词
D O I
10.1084/jem.178.4.1263
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Pro and pre B cells possess the long-term capacity to proliferate in vitro on stromal cells and interleukin 7 (IL-7) and can differentiate to surface immunoglobulin (sIg+) cells upon removal of IL-7 from the cultures. A key event in this differentiation is the extensive cell loss due to apoptosis. Because the proto-oncogene bcl-2 can promote cell survival, we established pre-B cell lines from Emu-bcl-2 transgenic mice. These pre-B cells have the same properties as those derived from non-bcl-2 transgenic mice except that they do not die by apoptosis. This allowed us to study the fate of newly formed B cells in vitro for a longer period of time. Here we show that early during the differentiation of pre-B cells, upregulation of RAG-1 and RAG-2 expression go hand in hand with rearrangements of the Ig gene loci. Moreover, the newly formed sIg+ B cells continue to express RAG-1 and RAG-2 and continue to rearrange L chain gene loci, even in the absence of proliferation, in an orderly fashion, so that kappaL+ sIg+ cells can become lambdaL+ sIg+ or sIg- cells, whereas lambdaL+ sIg+ cells can become sIg-, but not kappaL+ sIg+ cells. Thus, deposition of a complete Ig molecule on the surface of a B cell does not automatically stop the Ig-rearrangement machinery.
引用
收藏
页码:1263 / 1270
页数:8
相关论文
共 55 条
[1]   PREFERENTIAL LINKAGE OF BCL-2 TO IMMUNOGLOBULIN LIGHT CHAIN GENE IN CHRONIC LYMPHOCYTIC-LEUKEMIA [J].
ADACHI, M ;
TEFFERI, A ;
GREIPP, PR ;
KIPPS, TJ ;
TSUJIMOTO, Y .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 171 (02) :559-564
[2]   ACTIVITY OF MULTIPLE LIGHT CHAIN GENES IN MURINE MYELOMA CELLS PRODUCING A SINGLE, FUNCTIONAL LIGHT CHAIN [J].
ALT, FW ;
ENEA, V ;
BOTHWELL, ALM ;
BALTIMORE, D .
CELL, 1980, 21 (01) :1-12
[3]   CLONING THE CHROMOSOMAL BREAKPOINT OF T(14-18) HUMAN LYMPHOMAS - CLUSTERING AROUND JH ON CHROMOSOME-14 AND NEAR A TRANSCRIPTIONAL UNIT ON 18 [J].
BAKHSHI, A ;
JENSEN, JP ;
GOLDMAN, P ;
WRIGHT, JJ ;
MCBRIDE, OW ;
EPSTEIN, AL ;
KORSMEYER, SJ .
CELL, 1985, 41 (03) :899-906
[4]  
Berg J, 1990, Dev Immunol, V1, P53, DOI 10.1155/1990/56014
[5]   EXCLUSION AND INCLUSION OF ALPHA-T-CELL AND BETA-T-CELL RECEPTOR ALLELES [J].
BORGULYA, P ;
KISHI, H ;
UEMATSU, Y ;
VONBOEHMER, H .
CELL, 1992, 69 (03) :529-537
[6]  
BORZILLO GV, 1992, ONCOGENE, V7, P869
[7]   ENGAGEMENT OF THE T-CELL RECEPTOR DURING POSITIVE SELECTION IN THE THYMUS DOWN-REGULATES RAG-1 EXPRESSION [J].
BRANDLE, D ;
MULLER, C ;
RULICKE, T ;
HENGARTNER, H ;
PIRCHER, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) :9529-9533
[8]   B-CELL DEVELOPMENT IN MICE THAT LACK ONE OR BOTH IMMUNOGLOBULIN KAPPA-LIGHT CHAIN GENES [J].
CHEN, JZ ;
TROUNSTINE, M ;
KURAHARA, C ;
YOUNG, F ;
KUO, CC ;
XU, Y ;
LORING, JF ;
ALT, FW ;
HUSZAR, D .
EMBO JOURNAL, 1993, 12 (03) :821-830
[9]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[10]  
CLARKE S, 1991, J IMMUNOL, V146, P343