ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF THE FUN12 GENE OF SACCHAROMYCES-CEREVISIAE

被引：11

作者：

SUTRAVE, P ^{[1
]}

SHAFER, BK ^{[1
]}

STRATHERN, JN ^{[1
]}

HUGHES, SH ^{[1
]}

机构：

[1] NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,FREDERICK,MD 21702

来源：

GENE | 1994年 / 146卷 / 02期

关键词：

CLONING; DNA SEQUENCING; YEAST; GENE EXPRESSION; ESSENTIAL GENE;

D O I：

10.1016/0378-1119(94)90294-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and characterized the FUN12 gene which is found on chromosome I of Saccharomyces cerevisiae. The complete nucleotide (nt) sequence of the cDNA and the genomic clones shows that FUN12 is expressed as a 3.7-kb message and should encode a 97 kDa-protein. Immunoprecipitations using antipeptide antibodies showed that the cells contain a Fun12p of this size. The databases contain no nt sequences that are homologous to FUN12 and no protein homologous to Fun12p. Gene disruption experiments showed that FUN12 is an essential gene.

引用

页码：209 / 213

页数：5

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