REVERSIBLE TRANSLOCATION OF ANTIBIOTIC-RESISTANCE DETERMINANTS IN SALMONELLA-ORDONEZ

被引:13
作者
ROUSSEL, A
CARLIER, C
GERBAUD, G
CHABBERT, YA
CROISSANT, O
BLANGY, D
机构
[1] UNIV PIERRE & MARIE CURIE,INST RECH SCI CANC,VIRUS ONCOGENES LAB,F-94800 VILLEJUIF,FRANCE
[2] INST PASTEUR,SERV BACTERIOL MED,F-75724 PARIS,FRANCE
[3] INST PASTEUR,DEPT VIROL,F-75724 PARIS,FRANCE
来源
MOLECULAR & GENERAL GENETICS | 1979年 / 169卷 / 01期
关键词
D O I
10.1007/BF00267540
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Salmonella ordonez (BM 2000) codes for kanamycin (Km, aphA), ampicillin (Ap), streptomycin (SmSp: aadA and Sm: aphC), chloramphenicol (Cm), tetracycline (Tc) and sulfornamide (Su) resistances and for production of colicin Ib (Cib). Genetical analysis by incompatibility testing, conjugation, transformation and physical studies using electron microscopy, agarose gel electrophoresis, led us to associate the Km and Cib characters to a 98.7 kilobase (kb) IncII plasmid (pIP565), and the Sm (aphC) and Su determinants to a 8.3 kb plasmid (pIP605). The ApCmSmSp(aadA)SuTc determinants were not associated in BM2000 S. ordonez with a plasmid structure. Following conjugation of S. ordonez to E. coli, the ApCmSmSpSuTc determinants were found stably associated with a single plasmid structure (pIP173, 127.5 kb) belonging to IncII group. Agarose gel electrophoresis of plasmid DNA restriction endonuclease digests and electron microscopy heteroduplex analysis showed that the acquisition of the ApCmSmSpSuTc determinants resulted from the insertion into pIP565 of a 28.8 kb DNA sequence. This sequence coding for ApCmSmSpSuTc resistances in S. ordonez could be translocated either to pIP565 plasmid or to several IncII plasmids but never to plasmids belonging to IncW, IncP or IncFII, suggesting the existence of specific sequences on the IncII receptor plasmids. Mereover, R-determinants were translocated back en bloc" from pIP173 to the chromosome of a susceptible S. ordonez. The results were consistent with the presence in BM2000 S. ordonez chromosomal DNA of an integrated translocatable sequence encoding ApCmSmSpSuTc resistances. Such a structural association could account for the stability of these resistances in the Salmonella ordonez serotype. © 1979 Springer-Verlag."
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页码:13 / 25
页数:13
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