PHOTOSYNTHETIC ACTIVITY OF CYANOBACTERIA IN WATER-IN-OIL MICROEMULSIONS

被引:11
作者
FAMIGLIETTI, M [1 ]
HOCHKOPPLER, A [1 ]
WEHRLI, E [1 ]
LUISI, PL [1 ]
机构
[1] SWISS FED INST TECHNOL, INST ZELLBIOL, EM LAB 1, CH-8092 ZURICH, SWITZERLAND
关键词
CYANOBACTERIA; PHOTOSYNTHESIS; ORGANIC SOLVENT; MICROEMULSION; CELLS; MICROBIOLOGY;
D O I
10.1002/bit.260400124
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The solubilization and the photosynthetic activity of cyanobacteria (Anabaena variabilis) in water-in-oil microemulsions consisting of (Tween85/Span80)/hexadecane/water is investigated. Transparent and stable solutions containing up to 10(8) cells/mL could be obtained. The physical state and stability of the cells in the microemulsion, as evidenced from optical and electron microscopy, is dependent upon the physical parameters of the system, and in particular on the hydrophilic-lypophilic balance (HLB) of the surfactant system. Conditions could be found, under which the cells in the microemulsion system display photosynthetic activity. This was judged by measuring polarographically the oxygen evolution and by studying the photosynthetic activity in the presence of specific inhibitors.
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页码:173 / 178
页数:6
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