GENETIC AND BIOCHEMICAL-EVIDENCE FOR THE INVOLVEMENT OF THE COPROTEASE DOMAIN OF ESCHERICHIA-COLI RECA PROTEIN IN RECOMBINATION

被引：10

作者：

CAZAUX, C

DEFAIS, M

机构：

[1] Laboratoire de Pharmacologie et de Toxicologie Fondamentales, C.N.R.S., 31077 Toulouse Cedex

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1992年 / 223卷 / 04期

关键词：

RECA; RECOMBINATION; SOS REPAIR; STRAND-EXCHANGE REACTION; MUTAGENESIS;

D O I：

10.1016/0022-2836(92)90243-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RecA amino acid residue 204 is involved in the coprotease domain of the protein responsible for the induction of mutagenic repair. Two mutations were created at this site leading to the addition of either a methyl or an isopropyl group on the original glycine. Analyses of both the in vivo and the in vitro properties of these mutated proteins demonstrated that this residue 204 is involved in many RecA activities, suggesting that this site could allosterically direct conformational changes in the protein or could be situated in a region interacting with many RecA cofactors. © 1992.

引用

页码：823 / 829

页数：7

共 53 条

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