DETERMINATION OF A MONOCLONAL-ANTIBODY BINDING-ACTIVITY USING IMMUNODETECTION

A method for measuring the binding of chimeric IgG BR-96 to the Lewis Y antigen using the ImmunoDetection technology has been developed. The procedure is rapid (2 min), highly reproducible (<5% CV), and has excellent correlation with the Lewis Y and anti-idioty-pic enzyme immunoassays. Samples that were degraded by heat or by repeated freeze-thaw cycles showed reduced binding activity. There was minimal cross-reactivity with other proteins typically found in hybridoma media or with another chimeric IgG directed against a different antigen (L6). The fact that this assay can be performed using conventional HPLC equipment makes it especially attractive because it can be completely automated using equipment readily available. (C) 1994 Academic Press, Inc.