CLEAVAGE OF INTERCHAIN DISULFIDE BONDS FOLLOWING MATRIX-ASSISTED LASER-DESORPTION

被引：43

作者：

ZHOU, J

ENS, W

POPPESCHRIEMER, N

STANDING, KG

WESTMORE, JB

机构：

[1] UNIV MANITOBA,DEPT PHYS,WINNIPEG R3T 2N2,MANITOBA,CANADA

[2] UNIV MANITOBA,DEPT CHEM,WINNIPEG R3T 2N2,MANITOBA,CANADA

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES | 1993年 / 126卷

关键词：

DISULFIDE BRIDGES; LASER DESORPTION; TIME OF FLIGHT; UNIMOLECULAR DISSOCIATION;

D O I：

10.1016/0168-1176(93)80076-Q

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

The metastable decay of insulin ions produced by matrix-assisted laser desorption has been examined in a reflecting time-of-flight mass spectrometer. The [M-H]- molecular ion decays into negative A and B chain daughters with various numbers of sulfurs attached, corresponding to cleavage of the interchain disulfide bridges at different points. This type of decomposition can be identified by the characteristic 32 u spacing between the components.

引用

页码：115 / 122

页数：8

共 13 条

[1]

[Anonymous], NIST STANDARD REFERE

[2] CHARACTERIZATION OF DISULFIDE BOND POSITION IN PROTEINS AND SEQUENCE-ANALYSIS OF CYSTINE-BRIDGED PEPTIDES BY TANDEM MASS-SPECTROMETRY [J].