EXPRESSION OF HIV-1 GP120 AND HUMAN SOLUBLE CD4 BY RECOMBINANT BACULOVIRUSES AND THEIR INTERACTION INVITRO

被引:56
作者
MORIKAWA, Y
OVERTON, HA
MOORE, JP
WILKINSON, AJ
BRADY, RL
LEWIS, SJ
JONES, IM
机构
[1] NERC,INST VIROL,MANSFIELD RD,OXFORD OX1 3SR,ENGLAND
[2] CHESTER BEATTY LABS,INST CANC RES,LONDON SW3 6JB,ENGLAND
[3] UNIV YORK,DEPT CHEM,YORK YO1 5DD,N YORKSHIRE,ENGLAND
关键词
D O I
10.1089/aid.1990.6.765
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 × 109 cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported. © 1990, Mary Ann Liebert, Inc. All rights reserved.
引用
收藏
页码:765 / 773
页数:9
相关论文
共 25 条
  • [1] IDENTIFICATION OF THE RESIDUES IN HUMAN CD4 CRITICAL FOR THE BINDING OF HIV
    ARTHOS, J
    DEEN, KC
    CHAIKIN, MA
    FORNWALD, JA
    SATHE, G
    SATTENTAU, QJ
    CLAPHAM, PR
    WEISS, RA
    MCDOUGAL, JS
    PIETROPAOLO, C
    AXEL, R
    TRUNEH, A
    MADDON, PJ
    SWEET, RW
    [J]. CELL, 1989, 57 (03) : 469 - 481
  • [2] BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5
  • [3] SOLUBLE CD4 BLOCKS THE INFECTIVITY OF DIVERSE STRAINS OF HIV AND SIV FOR T-CELLS AND MONOCYTES BUT NOT FOR BRAIN AND MUSCLE-CELLS
    CLAPHAM, PR
    WEBER, JN
    WHITBY, D
    MCINTOSH, K
    DALGLEISH, AG
    MADDON, PJ
    DEEN, KC
    SWEET, RW
    WEISS, RA
    [J]. NATURE, 1989, 337 (6205) : 368 - 370
  • [4] THE CD4 (T4) ANTIGEN IS AN ESSENTIAL COMPONENT OF THE RECEPTOR FOR THE AIDS RETROVIRUS
    DALGLEISH, AG
    BEVERLEY, PCL
    CLAPHAM, PR
    CRAWFORD, DH
    GREAVES, MF
    WEISS, RA
    [J]. NATURE, 1984, 312 (5996) : 763 - 767
  • [5] A SOLUBLE FORM OF CD4 (T4) PROTEIN INHIBITS AIDS VIRUS-INFECTION
    DEEN, KC
    MCDOUGAL, JS
    INACKER, R
    FOLENAWASSERMAN, G
    ARTHOS, J
    ROSENBERG, J
    MADDON, PJ
    AXEL, R
    SWEET, RW
    [J]. NATURE, 1988, 331 (6151) : 82 - 84
  • [6] MODEL FOR INTRACELLULAR FOLDING OF THE HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 GP120
    FENNIE, C
    LASKY, LA
    [J]. JOURNAL OF VIROLOGY, 1989, 63 (02) : 639 - 646
  • [7] A SOLUBLE CD4 PROTEIN SELECTIVELY INHIBITS HIV REPLICATION AND SYNCYTIUM FORMATION
    HUSSEY, RE
    RICHARDSON, NE
    KOWALSKI, M
    BROWN, NR
    CHANG, HC
    SILICIANO, RF
    DORFMAN, T
    WALKER, B
    SODROSKI, J
    REINHERZ, EL
    [J]. NATURE, 1988, 331 (6151) : 78 - 81
  • [8] LYMPHOCYTE-T T4 MOLECULE BEHAVES AS THE RECEPTOR FOR HUMAN RETROVIRUS LAV
    KLATZMANN, D
    CHAMPAGNE, E
    CHAMARET, S
    GRUEST, J
    GUETARD, D
    HERCEND, T
    GLUCKMAN, JC
    MONTAGNIER, L
    [J]. NATURE, 1984, 312 (5996) : 767 - 768
  • [9] DELINEATION OF A REGION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 GP120 GLYCOPROTEIN CRITICAL FOR INTERACTION WITH THE CD4 RECEPTOR
    LASKY, LA
    NAKAMURA, G
    SMITH, DH
    FENNIE, C
    SHIMASAKI, C
    PATZER, E
    BERMAN, P
    GREGORY, T
    CAPON, DJ
    [J]. CELL, 1987, 50 (06) : 975 - 985
  • [10] CORRECTED CD4 SEQUENCE
    LITTMAN, DR
    MADDON, PJ
    AXEL, R
    [J]. CELL, 1988, 55 (04) : 541 - 541