LARGE-SCALE PREPARATION OF PURE PLASTOCYANIN FROM SPINACH

被引：12

作者：

MORAND, LZ ^{[1
]}

KROGMANN, DW ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1141卷 / 01期

基金：

美国国家科学基金会;

关键词：

PLASTOCYANIN; CYTOCHROME-F; CHROMATOGRAPHY;

D O I：

10.1016/0005-2728(93)90195-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

50 mg of pure plastocyanin was prepared from 5 kg spinach leaves in four steps: (1) acetone extraction; 2 ammonium sulfate fractionation; (3) Sepharose column chromatography; and (4) concentration. The acetone and ammonium sulfate steps allowed for the use of a single chromatographic step which yielded plastocyanin free of contaminants.

引用

页码：105 / 106

页数：2

共 8 条

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