THE ORIENTATION OF PORIN OMPF IN THE OUTER-MEMBRANE OF ESCHERICHIA-COLI

被引:38
作者
HOENGER, A
PAGES, JM
FOUREL, D
ENGEL, A
机构
[1] UNIV BASEL, BIOCTR, ME MULLER INST HIGH RESOLUT ELECTRON MICROSCOPY, KLINGELBERGSTR 70, CH-4056 BASEL, SWITZERLAND
[2] CNRS, GDR 1000, UPR 9027, F-13402 MARSEILLE 9, FRANCE
关键词
BACTERIAL OUTER MEMBRANE; OMPF PORIN; SURFACE EPITOPES; COLICINS; IMMUNOGOLD LABELING;
D O I
10.1006/jmbi.1993.1520
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The in vivo orientation of the channel forming porin OmpF from the outer membrane of Escherichia coli was assessed by immunological, biochemical and structural techniques. Porin OmpF exists as a trimer of channels formed by 16 antiparallel β-strands. These are connected by long hydrophilic loops on one side of the bilayer and short loops or β-turns on the other. The former constitute the rough side of the porin channel, the latter the smooth side. Epitopes at the cell surface have all been mapped within the long loops, suggesting a rough-side-out orientation of OmpF in the membrane. We analyzed detergent solubilized OmpF trimers, reconstituted 2-D OmpF crystals, OmpF containing outer membranes (sacculi) and intact cells of an E. coli strain overexpressing OmpF. Both solubilized OmpF and OmpF containing sacculi were exposed to proteases, and distinct cleavage sites were identified by protein sequencing. Solubilized OmpF, reconstituted 2-D OmpF crystals and detergent extracted sacculi were tested for their capacity to adsorb colicin N. We used antibodies directed against surface exposed epitopes for immunogold labeling of reconstituted 2-D OmpF crystals and sacculi. The surfaces of intact cells and extracted sacculi were analyzed by electron microscopy and image processing. Finally, a full 3-D reconstruction of negatively stained OmpF containing sacculi revealed the OmpF trimer in its native conformation within the outer membrane. Colicin N and antibody experiments, as well as the 3-D map of the sacculi demonstrated that OmpF exposes the long loops to the extracellular space. In contrast, reconstituted crystalline OmpF vesicles and double layered sheets were found to be in an inside-out conformation, hence hiding colicin or antibody binding epitopes. Two proteinase K cleavage sites were identified, one on a protruding loop and the other inside the channel on the loop penetrating the pore. © 1993 Academic Press Limited.
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页码:400 / 413
页数:14
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