A NEW ATP-INDEPENDENT DNA ENDONUCLEASE FROM SCHIZOSACCHAROMYCES-POMBE THAT RECOGNIZES CYCLOBUTANE PYRIMIDINE DIMERS AND 6-4-PHOTOPRODUCTS

被引：85

作者：

BOWMAN, KK

SIDIK, K

SMITH, CA

TAYLOR, JS

DOETSCH, PW

FREYER, GA

机构：

[1] COLUMBIA UNIV,COLL PHYSICIANS & SURGEONS,CTR RADIOL RES,NEW YORK,NY 10032

[2] EMORY UNIV,SCH MED,DEPT BIOCHEM,ATLANTA,GA 30322

[3] EMORY UNIV,SCH MED,DEPT RADIAT ONCOL,ATLANTA,GA 30322

[4] WASHINGTON UNIV,DEPT CHEM,ST LOUIS,MO 63130

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 15期

关键词：

D O I：

10.1093/nar/22.15.3026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have discovered a new DNA endonuclease in the fission yeast Schizosaccharomyces pombe which recognizes cyclobutane pyrimidine dimers and (6-4) pyrimidine-pyrimidone photoproducts. S. pombe DNA endonuclease (SPDE) catalyzes a single ATP-independent incision immediately 5' to the UV photoproduct and generates termini containing 3' hydroxyl and 5' phosphoryl groups. Based on these properties, we propose that SPDE may function in a DNA repair capacity, representing the initial recognition/cleavage step of a DNA excision repair pathway.

引用

页码：3026 / 3032

页数：7

共 30 条

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