EFFECT OF NITROGEN AND SUCROSE ON THE PRIMARY AND SECONDARY METABOLISM OF TRANSFORMED ROOT CULTURES OF HYOSCYAMUS-MUTICUS

被引:42
作者
OKSMANCALDENTEY, KM
SEVON, N
VANHALA, L
HILTUNEN, R
机构
[1] Pharmacognosy Division, Department of Pharmacy, University of Helsinki, FIN-00014
关键词
FATTY ACIDS; HYOSCYAMINE; HYOSCYAMUS MUTICUS; NITROGEN; SUCROSE; TRANSFORMED ROOT CULTURES;
D O I
10.1007/BF00033886
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of carbon and nitrogen sources on two well-established hairy root clones, LBA1S and C58A, of Hyoscyamus muticus strain Cairo, were investigated. Both clones exhibited completely different patterns with regards to their growth rate, hyoscyamine accumulation, and fatty acid contents. Clone C58A grew faster and yielded more biomass (17.4 g 1(-1), in 21 days), but produced less hyoscyamine. The maximum hyoscyamine content (120 mg 1(-1)) in clone LBA1S was reached in 28 days. Neither of the clones could use lactose or fructose as the sole carbon source, nor ammonium as the sole nitrogen source. The growth in the medium containing glucose was significantly reduced compared to that containing sucrose. Clone LBA1S was sensitive to the changes in sucrose concentration and an increase in ammonium in the culture medium, whereas C58A tolerated these changes better but was more sensitive to the increase in total nitrogen. Lipid synthesis was active in the exponential growth phase, and the total fatty acid content varied from 5 to 34 mg g(-1) of dry root material. The major fatty acids were linoleic, palmitic and linolenic. There were considerable differences in the total amount of lipids and in their relative ratios when different nutrients were applied.
引用
收藏
页码:263 / 272
页数:10
相关论文
共 28 条
  • [1] ALKALOIDS AND LIPIDS OF HETEROTROPHIC, PHOTOMIXOTROPHIC AND PHOTOAUTOTROPHIC CELL-SUSPENSION CULTURES OF PEGANUM HARMALA
    BARZ, W
    HERZBECK, H
    HUSEMANN, W
    SCHNEIDERS, G
    MANGOLD, HK
    [J]. PLANTA MEDICA, 1980, 40 (02) : 137 - 148
  • [2] CHRISTEN P, 1992, PLANT CELL REP, V11, P597, DOI 10.1007/BF00236380
  • [3] FOWLER MW, 1982, J CHEM TECHNOL BIOT, V32, P338
  • [4] FRIED J, 1991, HDB THIN LAYER CHROM, V55, P593
  • [5] NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS
    GAMBORG, OL
    MILLER, RA
    OJIMA, K
    [J]. EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) : 151 - +
  • [6] HUSEMANN W, 1985, CELL CULTURE SOMATIC, V2, P213
  • [7] IMPROVEMENT OF THE CATHARANTHINE PRODUCTIVITY IN HAIRY ROOT CULTURES OF CATHARANTHUS-ROSEUS BY USING MONOSACCHARIDES AS A CARBON SOURCE
    JUNG, KH
    KWAK, SS
    KIM, SW
    LEE, H
    CHOI, CY
    LIU, JR
    [J]. BIOTECHNOLOGY LETTERS, 1992, 14 (08) : 695 - 700
  • [8] KUNZ WGW, 1985, CRC CRIT R BIOTECH, V2, P105
  • [9] KURZ WGW, 1985, CRC CR REV BIOTECHN, V2, P105
  • [10] FATTY-ACID COMPOSITION AND BIOSYNTHESIS IN CELL-SUSPENSION CULTURES OF GLYCINE-MAX (L) MERR, CATHARANTHUS-ROSEUS G DON AND NICOTIANA-TABACUM-L
    MACCARTHY, JJ
    STUMPF, PK
    [J]. PLANTA, 1980, 147 (05) : 384 - 388