MEASURING H-TYPE-1 AND H-TYPE-2 ANTIGENS IN HUMAN SALIVA BY IMMUNOASSAY USING ARTIFICIAL ANTIGENS AS STANDARD SUBSTANCES

被引:15
作者
WANG, BJ [1 ]
AKIYAMA, K [1 ]
JIA, JT [1 ]
KIMURA, H [1 ]
机构
[1] KURUME UNIV,SCH MED,DEPT LEGAL MED,KURUME,FUKUOKA 830,JAPAN
关键词
H TYPE 1; H TYPE 2; HUMAN SALIVA; IMMUNOASSAY;
D O I
10.1016/0379-0738(94)90405-7
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
A time-resolved fluorometric immunoassay for water-soluble H antigens of ABH blood group substances has been developed. Introducing artificial antigens (trisaccharide-albumin complexes for H type 1 and H type 2) as reference substances, H blood group substances in human saliva were measured. The monoclonal antibody, anti-H 1E3, reacted with both H type 1 and type 2 chains, and the commercially available anti-H reacted with H type 2 chain. Using these two antibodies we found 10-20-fold higher concentration of H type 1 compared with that of H type 2 in human saliva. The H type 2 was not found in the saliva samples from nonsecretors, irrespective of ABO phenotypes. The results suggest that similar to 90% of A and B blood group substances in human saliva are built on type 1 chains.
引用
收藏
页码:1 / 8
页数:8
相关论文
共 25 条
[1]  
BAIG MM, 1973, J IMMUNOL, V111, P1826
[2]   GENERAL PRINCIPLES OF RADIOIMMUNOASSAY [J].
BERSON, SA ;
YALOW, RS .
CLINICA CHIMICA ACTA, 1968, 22 (01) :51-&
[3]  
BJORK S, 1987, J BIOL CHEM, V262, P6758
[4]  
BOLTON S, 1986, J FORENSIC SCI, V31, P27
[5]   SYNTHESIS OF POLYMERIC NEOGLYCOCONJUGATES BASED ON N-SUBSTITUTED POLYACRYLAMIDES [J].
BOVIN, NV ;
KORCHAGINA, EY ;
ZEMLYANUKHINA, TV ;
BYRAMOVA, NE ;
GALANINA, OE ;
ZEMLYAKOV, AE ;
IVANOV, AE ;
ZUBOV, VP ;
MOCHALOVA, LV .
GLYCOCONJUGATE JOURNAL, 1993, 10 (02) :142-151
[6]   ABH AND RELATED HISTO-BLOOD GROUP ANTIGENS - IMMUNOCHEMICAL DIFFERENCES IN CARRIER ISOTYPES AND THEIR DISTRIBUTION [J].
CLAUSEN, H ;
HAKOMORI, S .
VOX SANGUINIS, 1989, 56 (01) :1-20
[7]   A-ACTIVE TRISACCHARIDES ISOLATED FROM A1 AND A2 BLOOD-GROUP-SPECIFIC GLYCOPROTEINS [J].
DONALD, ASR .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1981, 120 (02) :243-249
[9]  
HOLBURN AM, 1974, BRIT J HAEMATOL, V28, P157
[10]   THE IMPACT OF MONOCLONAL-ANTIBODIES IN BLOOD-GROUP SEROLOGY [J].
ISSITT, PD .
TRANSFUSION, 1989, 29 (01) :58-64