USE OF A SOLID-PHASE PHOTOAFFINITY REAGENT TO LABEL A STEROID BINDING-SITE - APPLICATION TO THE DELTA-5-3-KETOSTEROID ISOMERASE OF PSEUDOMONAS-TESTOSTERONI

被引：17

作者：

HEARNE, M ^{[1
]}

BENISEK, WF ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,SCH MED,DEPT BIOL CHEM,DAVIS,CA 95616

来源：

BIOCHEMISTRY | 1985年 / 24卷 / 26期

关键词：

D O I：

10.1021/bi00347a001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：7511 / 7516

页数：6

共 20 条

[1]

Benisek W F, 1977, Methods Enzymol, V46, P469

[2] AFFINITY CHROMATOGRAPHY OF 3-OXOSTEROID DELTA4-DELTA5-ISOMERASE OF PSEUDOMONAS-TESTOSTERONI [J].

BENSON, AM ;

SURUDA, AJ ;

SHAW, R ;

TALALAY, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1974, 348 (02) :317-320

[3]

BENSON AM, 1971, J BIOL CHEM, V246, P7514

[4] MODIFICATION OF AN ENZYME CARBOXYLATE RESIDUE IN THE INHIBITION OF 3-OXO-DELTA-5-STEROID ISOMERASE BY (3S)-SPIRO[5-ALPHA-ANDROSTANE-3,2'-OXIRANE]-17-BETA-OL - IMPLICATIONS FOR THE MECHANISM OF ACTION [J].

BEVINS, CL ;

BANTIA, S ;

POLLACK, RM ;

BOUNDS, PL ;

KAYSER, RH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (17) :4957-4962

[5]

BHOWN AS, 1978, J CHROMATOGR, V148, P532, DOI 10.1016/S0021-9673(00)85319-4

[6] THE DIRECT CONVERSION OF STEROIDAL DELTA-5-3-BETA-ALCOHOLS TO DELTA-5-KETONES AND DELTA-4-3-KETONES [J].

DJERASSI, C ;

ENGLE, RR ;

BOWERS, A .

JOURNAL OF ORGANIC CHEMISTRY, 1956, 21 (12) :1547-1548

[7] MODIFICATIONS OF DELTA-5-3-KETOSTEROID ISOMERASE INDUCED BY ULTRAVIOLET-IRRADIATION IN THE PRESENCE OF THE SOLID-PHASE PHOTOAFFINITY REAGENT DELTA-6-TESTOSTERONE AGAROSE [J].

HEARNE, M ;

BENISEK, WF .

JOURNAL OF PROTEIN CHEMISTRY, 1984, 3 (01) :87-97

[8] PHOTOAFFINITY MODIFICATION OF DELTA-5-3-KETOSTEROID ISOMERASE BY LIGHT-ACTIVATABLE STEROID KETONES COVALENTLY COUPLED TO AGAROSE BEADS [J].

HEARNE, M ;

BENISEK, WF .

BIOCHEMISTRY, 1983, 22 (10) :2537-2544

[9]

HUNKAPILLER MW, 1983, METHOD ENZYMOL, V91, P486

[10]

JARABAK R, 1969, METHOD ENZYMOL, V15, P642

← 1 2 →