ISOLATION OF AN ACTIVATOR FOR PROSTAGLANDIN HYDROPEROXIDASE FROM BOVINE VESICULAR GLAND CYTOSOL AND ITS IDENTIFICATION AS URIC-ACID

被引：45

作者：

OGINO, N

YAMAMOTO, S

HAYAISHI, O

TOKUYAMA, T

机构：

[1] KYOTO UNIV,FAC MED,DEPT MED CHEM,SAKYO KU,KYOTO 606,JAPAN

[2] OSAKA CITY UNIV,FAC SCI,DEPT CHEM,OSAKA 558,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 87卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91664-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzymatic conversion of prostaglandin G1 to H1 was stimulated by an activator present in the cytosol of bovine vesicular gland. The activator was purified by Sephadex G-25 gel filtration and Dowex 1 column chromatography. The purified activator was identified to be uric acid by thin layer chromatography, ultraviolet and infrared absorption spectroscopy and combined gas chromatography-mass spectroscopy. Among various purine compounds tested, only uric acid and 2,8-dihydroxyadenine were active. © 1979.

引用

页码：184 / 191

页数：8

共 15 条

[1]

Ames B., 1966, METHOD ENZYMOL, V8, P115, DOI DOI 10.1016/0076-6879(66)08014-5

[2] ISOLATION AND STRUCTURE OF 2 PROSTAGLANDIN ENDOPEROXIDES THAT CAUSE PLATELET-AGGREGATION [J].