RETROVIRUS GAG PROTEIN P-30 IN THE ISLETS OF NONOBESE DIABETIC MICE - RELEVANCE FOR PATHOGENESIS OF DIABETES-MELLITUS

被引：23

作者：

NAKAGAWA, C ^{[1
]}

HANAFUSA, T ^{[1
]}

MIYAGAWA, J ^{[1
]}

YUTSUDO, M ^{[1
]}

NAKAJIMA, H ^{[1
]}

YAMAMOTO, K ^{[1
]}

TOMITA, K ^{[1
]}

KONO, N ^{[1
]}

HAKURA, A ^{[1
]}

TARUI, S ^{[1
]}

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,DEPT TUMOUR VIROL,OSAKA,JAPAN

来源：

DIABETOLOGIA | 1992年 / 35卷 / 07期

关键词：

NONOBESE DIABETIC (NOD) MICE; RETROVIRUS; GAG PROTEIN P-30; AUTOIMMUNITY; CYCLOPHOSPHAMIDE; PATHOGENESIS; WESTERN BLOT ANALYSIS; ULTRASTRUCTURE;

D O I：

10.1007/BF00400251

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We investigated the presence of retroviral protein in the pancreatic islets of non-obese diabetic mice to prove that the virus-like particle observed specifically in the pancreatic Beta cell of these mice was retrovirus. Western blot analysis probed with anti-retrovirus antibody demonstrated the existence of retroviral gag (group specific antigen) protein p30 in the islets of female non-obese diabetic mice. Islets of non-obese diabetic mice which were treated with cyclophosphamide, known to accelerate the development of insulitis and diabetes mellitus, have shown both a significantly increased number of retrovirus-like particles (type C) and enhanced expression of gag protein p30, compared to those of mice not treated with cyclophosphamide. These results confirmed the presence of type C retrovirus in non-obese diabetic mouse Beta cells and suggest a role for retrovirus in the development of insulitis and diabetes in these mice.

引用

页码：614 / 618

页数：5

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