SELECTION AND EVALUATION OF ASTAXANTHIN-OVERPRODUCING MUTANTS OF PHAFFIA-RHODOZYMA

被引：61

作者：

MEYER, PS ^{[1
]}

DUPREEZ, JC ^{[1
]}

KILIAN, SG ^{[1
]}

机构：

[1] UNIV ORANGE FREE STATE,DEPT MICROBIOL & BIOCHEM,POB 339,BLOEMFONTEIN 9300,SOUTH AFRICA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1993年 / 9卷 / 05期

关键词：

ASTAXANTHIN; MANNITOL; MUTANTS; NTG; PHAFFIA-RHODOZYMA; SUCCINATE; VALINE; YEAST;

D O I：

10.1007/BF00386286

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Mutagenesis of Phaffia rhodozyma with NTG yielded a mutant with an astaxanthin content of 1688 mug (g dry biomass)-1, a cell yield coefficient of 0.4 7 on glucose and a maximum specific growth rate of 0.12 h-1. Re-mutation of the mutant decreased the cell yield and maximum specific growth rate but increased the astaxanthin content. The use of mannitol or succinate as carbon sources enhanced pigmentation, yielding astaxanthin contents of 1973 mug g-1 and 1926 mug g-1, respectively. The use of valine as sole nitrogen source also increased astaxanthin production, but severely decreased the maximum specific growth rate and cell yield coefficient. The optimum pH for growth of P. rhodozyma was between pH 4.5 and 5.5, whereas the astaxanthin content remained constant above pH 3.

引用

页码：514 / 520

页数：7

共 19 条

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