CONNECTIN, GIANT ELASTIC PROTEIN, IN GIANT SARCOMERES OF CRAYFISH CLAW MUSCLE

被引：33

作者：

MANABE, T

KAWAMURA, Y

HIGUCHI, H

KIMURA, S

MARUYAMA, K

机构：

[1] CHIBA UNIV,FAC SCI,DEPT BIOL,CHIBA,CHIBA 263,JAPAN

[2] JIKEI UNIV,SCH MED,DEPT PHYSIOL,TOKYO,TOKYO 105,JAPAN

来源：

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY | 1993年 / 14卷 / 06期

关键词：

D O I：

10.1007/BF00141562

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In the giant sarcomeres (sarcomere length, 10 mu m at rest) of crayfish claw muscle, 3000 kDa connectin-like protein but not projectin (mini-titin) appears to be responsible for passive tension generation. Proteolysis of crayfish connectin in skinned fibres was parallel with disappearance of resting tension. Immunofluorescence observations using the antiserum to crayfish connectin showed that crayfish connectin linked the A band to the Z line in a giant sarcomere. It appears that crayfish connectin exerts a centering force on the A band in a sarcomere. Very thin filaments in the I band were visualized after the actin filaments had been removed by the treatment with plasma gelsolin. Crayfish connectin was partially purified and its rotary shadowed image was a very long filament. Projectin was localized on the A band of crayfish giant sarcomeres and remained unmoved during stretch or contraction. However, on dissolution of myosin filaments, projectin moved to the Z line together with crayfish connectin. It seems that projectin binds to connectin on the myosin filament. In regular size of sarcomeres (sarcomere lengths, 3-4 mu m at rest) of crayfish stretcher muscle, projectin linked the A band to the Z line, as in insect flight muscle.

引用

页码：654 / 665

页数：12

共 22 条

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