DIRECT MEASUREMENT OF TEMPERATURE-CHANGES WITHIN FREEZE-FRACTURE SPECIMENS DURING RAPID QUENCHING IN LIQUID COOLANTS

被引：166

作者：

COSTELLO, MJ

CORLESS, JM

机构：

[1] Department of Anatomy, Duke University Medical Center, Durham, North Carolina, 27710

来源：

JOURNAL OF MICROSCOPY | 1978年 / 112卷 / JAN期

关键词：

D O I：

10.1111/j.1365-2818.1978.tb01151.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We have evaluated the cooling rates of specimens mounted in a variety of freeze‐fracture holders when plunged into a series of liquid coolants. These rates were measured using miniature thermocouples placed within the mounted specimens. The most rapid cooling rates were obtained using propane at 83 K as the coolant. When mounted on a newly devised ‘copper sandwich’ holder, specimen cooling rates in excess of 4500 K/s have been recorded. A simple guillotine‐like device for quenching freeze‐fracture specimens under reproducible conditions is presented. 1978 Blackwell Science Ltd

引用

页码：17 / 37

页数：21

共 27 条

[1] CORRELATED X-RAY-DIFFRACTION AND FREEZE-FRACTURE STUDIES ON MEMBRANE MODEL SYSTEMS PERTURBATIONS INDUCED BY FREEZE-FRACTURE PREPARATIVE PROCEDURES [J].