STRETCH-ACTIVATED COMPOSITE ION CHANNELS IN BACILLUS-SUBTILIS

被引：35

作者：

ZORATTI, M

PETRONILLI, V

SZABO, I

机构：

[1] Centro CNR Fisiologia Mitocondri, Istituto di Patologia Generale, Dipartimento di Biologia, 35121 Padova

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92341-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The presence of ion-conducting pores in the membrane of Bacillus subtilis giant protoplasts was discovered using the patch-clamp technique. Membrane stretch caused the activation of several conductances with values in the nS range. The observations indicate the presence of substate levels and of aggregates of channels behaving in a cooperative manner. Following repeated stretch cycles, the channels exhibited spontaneous activity. The characteristics of the electrical phenomena afterwards changed in time in a manner suggesting the decay of the giant channels into lower-conductance species, presumably corresponding to building blocks of the giant stretch-activated channels. © 1990.

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页码：443 / 450

页数：8

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