RELATIONSHIP BETWEEN CHANGES IN THE CALCIUM DEPENDENT REGULATORY PROTEIN AND ADENYLATE-CYCLASE DURING VIRAL TRANSFORMATION

被引：57

作者：

LAPORTE, DC ^{[1
]}

GIDWITZ, S ^{[1
]}

WEBER, MJ ^{[1
]}

STORM, DR ^{[1
]}

机构：

[1] UNIV ILLINOIS,DEPT MICROBIOL,URBANA,IL 61801

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 04期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0006-291X(79)90240-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The levels of the calcium dependent regulatory protein in transformed chicken embryo fibroblasts are higher both in soluble fractions and membrane fractions compared to untransformed cells. The kinetics for changes in the calcium dependent regulatory protein, hexose transport, and adenylate cyclase were compared using a temperature sensitive mutant of Rous sarcoma virus. Decreases in adenylate cyclase activity and increased hexose transport accompanying transformation occurred with half-lives of approximately 7 to 8 hours. Increases in the calcium dependent regulatory protein occurred much slower with a half-life of seventeen hours. It is concluded that the increase in calcium dependent regulatory protein levels is a late event during viral transformation and that the decline in adenylate cyclase activity cannot be due to changes in the amount of calcium dependent regulatory protein. © 1979.

引用

页码：1169 / 1177

页数：9

共 11 条

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