OBSERVATIONS ON THE CHEMICAL NATURE OF LEAD IN HUMAN BLOOD-SERUM

被引：38

作者：

ALMODHEFER, AJA ^{[1
]}

BRADBURY, MWB ^{[1
]}

SIMONS, TJB ^{[1
]}

机构：

[1] UNIV LONDON KINGS COLL,DIV BIOMED SCI,PHYSIOL GRP,LONDON WC2R 2LS,ENGLAND

来源：

CLINICAL SCIENCE | 1991年 / 81卷 / 06期

关键词：

BLOOD; LEAD; SERUM;

D O I：

10.1042/cs0810823

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

1. The binding of lead to human blood serum, and components of serum, was studied by titration with the addition of Pb(NO3)2 solution, monitoring the free Pb2+ concentration with a Pb2+ electrode, and by equilibrium dialysis. 2. In fresh serum, about 4999 out of 5000 parts of added lead were bound. This suggests that the free Pb2+ concentration is around 1/5000th of the total lead concentration in the serum of normal subjects, i.e. about 1 x 10(-12) mol/l. 3. About 60% of the binding of lead in serum is abolished by standing in air, by dialysis or by treatment with N-ethylmaleimide. This appears to be due to the presence of thiol compounds, mainly cysteine. The remaining 40% appears to be due to protein, mainly albumin.

引用

页码：823 / 829

页数：7

共 19 条

[1]

ALMODHEFER AJA, 1990, J PHYSIOL-LONDON, V522, pP56

[2] STUDY ON LEAD HYDROXIDE EQUILIBRIUM BY USE OF SELECTIVE ELECTRODES [J].

BIRRAUX, C ;

LANDRY, JC ;

HAERDI, W .

ANALYTICA CHIMICA ACTA, 1977, 93 (01) :281-285

[3]

DESILVA PE, 1981, BRIT J IND MED, V38, P209

[4] A SPECTROPHOTOMETRIC METHOD FOR DIRECT DETERMINATION OF CYSTEINE IN PRESENCE OF OTHER NATURALLY OCCURRING AMINO ACIDS [J].

GAITONDE, MK .

BIOCHEMICAL JOURNAL, 1967, 104 (02) :627-&

[5] A MICRO BIURET METHOD FOR PROTEIN DETERMINATION - DETERMINATION OF TOTAL PROTEIN IN CEREBROSPINAL FLUID [J].

GOA, J .

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 1953, 5 (03) :218-222

[6] STUDIES ON THE PLASMA PROTEINS .105. PREPARATION AND PROPERTIES OF SERUM AND PLASMA PROTEINS .39. THE INTERACTION OF HUMAN SERUM ALBUMIN WITH PLUMBOUS IONS [J].

GURD, FRN ;

MURRAY, GR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1954, 76 (01) :187-190

[7] EQUILIBRIUM STUDIES ON THE BINDING OF CADMIUM(II) TO HUMAN-SERUM TRANSFERRIN [J].

HARRIS, WR ;

MADSEN, LJ .

BIOCHEMISTRY, 1988, 27 (01) :284-288

[8] THERMODYNAMIC BINDING CONSTANTS OF THE ZINC HUMAN-SERUM TRANSFERRIN COMPLEX [J].

HARRIS, WR .

BIOCHEMISTRY, 1983, 22 (16) :3920-3926

[9]

HERNBERG S, 1980, LEAD TOXICITY, P367

[10] EVALUATION OF SOME COMMERCIAL LEAD(II)-SELECTIVE ELECTRODES [J].

KIVALO, P ;

VIRTANEN, R ;

WICKSTROM, K ;

WILSON, M ;

PUNGOR, E ;

HORVAI, G ;

TOTH, K .

ANALYTICA CHIMICA ACTA, 1976, 87 (02) :401-409

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