EVALUATION OF CELLULAR MECHANISMS FOR MODULATION OF CALCIUM TRANSIENTS USING A MATHEMATICAL-MODEL OF FURA-2 CA-2+ IMAGING IN APLYSIA SENSORY NEURONS

被引：34

作者：

BLUMENFELD, H

ZABLOW, L

SABATINI, B

机构：

[1] Center for Neurobiology and Behavior, Howard Hughes Medical Institute, Columbia University, New York, New York

来源：

BIOPHYSICAL JOURNAL | 1992年 / 63卷 / 04期

关键词：

D O I：

10.1016/S0006-3495(92)81670-3

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A theoretical model of [Ca++]i diffusion, buffering, and extrusion was developed for Aplysia sensory neurons, and integrated with the measured optical transfer function of our fura-2 microscopic recording system, in order to fully simulate fura-2 video or photomultiplier tube measurements of [Ca++]i. This allowed an analysis of the spatial and temporal distortions introduced during each step of fura-2 measurements of [Ca++]i in cells. In addition, the model was used to evaluate the plausibility of several possible mechanisms f or modulating [Ca++]i transients evoked by action potentials. The results of the model support prior experimental work (Blumenfeld, Spira, Kandel, and Siegelbaum, 1990. Neuron. 5: 487-499), suggesting that 5-HT and FMRFamide modulate action potential-induced [Ca++]i transients in Aplysia sensory neurons through changes in Ca++ influx, and not through changes in [Ca++]i homeostasis or release from internal stores.

引用

页码：1146 / 1164

页数：19

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