THE ACCESSORY COLONIZATION FACTOR AND TOXIN-COREGULATED PILUS GENE CLUSTERS ARE PHYSICALLY LINKED ON THE VIBRIO-CHOLERAE-0395 CHROMOSOME

被引:19
作者
EVERISS, KD [1 ]
HUGHES, KJ [1 ]
PETERSON, KM [1 ]
机构
[1] LOUISIANA STATE UNIV,MED CTR,DEPT MICROBIOL & IMMUNOL,SHREVEPORT,LA 71130
来源
DNA SEQUENCE | 1994年 / 5卷 / 01期
关键词
ACCESSORY COLONIZATION FACTOR; ACFB GENE; TCPJ GENE; TOXIN-COREGULATED PILUS; VIBRIO CHOLERAE;
D O I
10.3109/10425179409039704
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A partial nucleotide sequence of the first gene in the Vibrio cholerae accessory colonization factor gene cluster was obtained by sequencing a cloned acfB::TnphoA fusion junction. Analysis of the sequence data revealed that the ATC start codon of the acfB structural gene is located 6 bp downstream of the tcpJ TCA stop codon. This establishes a large region in excess of 20 kbp of the Vibrio cholerae chromosome as a cluster of coordinately regulated virulence genes involved in intestinal colonization. The absence of a characteristic transcription termination site in the tcpJ-acfB intergenic region indicates that acfB may be encoded on the same messenger RNA as tcpJ. The deduced amino acid sequence for the N-terminal 143 amino acids of AcfB reveals a po- tential leader peptide, that lacks a consensus signal peptidase I recognition sequence. Computer algorithms fail to detect any significant similarities between the partial AcfB amino acid sequence and any entries in the SWISS-PROT database.
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页码:51 / 55
页数:5
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