BETA-GUANIDINOPROPIONATE AND PHOSPHORYLATED BETA-GUANIDINOPROPIONATE AS SUBSTRATES FOR CREATINE-KINASE

被引：58

作者：

CHEVLI, R

FITCH, CD

机构：

[1] Department of Internal Medicine, St. Louis University School of Medicine, St. Louis

来源：

BIOCHEMICAL MEDICINE | 1979年 / 21卷 / 02期

关键词：

D O I：

10.1016/0006-2944(79)90068-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rabbit muscle creatine kinase catalyzes transphosphorylation between ATP and β-guanidinopropionate at pH 9 and 23°C with a Km for β-guanidinopropionate of 50 mm and a Vmax of 0.21 μmole/min/mg of creatine kinase. The comparable values for the phosphorylation of creatine are 16.7 for Km and 75.1 for Vmax. In the reverse direction at pH 7.05 and 23°C, phosphorylated β-guanidinopropionate has a Km of 4.9 and a Vmax of 0.22 in comparison to a Km of 2.2 and a Vmax of 231 for phosphocreatine. © 1979.

引用

页码：162 / 167

页数：6

共 19 条

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