LARGE-SCALE PREPARATION OF HIGHLY PURIFIED PYROGEN-FREE HUMAN GROWTH-HORMONE FOR CLINICAL USE

被引：38

作者：

JONES, RL ^{[1
]}

BENKER, G ^{[1
]}

SALACINSKI, PR ^{[1
]}

LLOYD, TJ ^{[1
]}

LOWRY, PJ ^{[1
]}

机构：

[1] MED KLIN ESSEN,D-43 ESSEN,FED REP GER

来源：

JOURNAL OF ENDOCRINOLOGY | 1979年 / 82卷 / 01期

关键词：

D O I：

10.1677/joe.0.0820077

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A method is described for the large isolation of highly purified human growth hormone for therapeutic use. The hormone was extracted from frozen pituitary glands under mildly alkaline conditions. Purification was effected by gel filtration and ion-exchange chromatography. The final product was pyrogen-free and had a potency of 2·5 i.u./mg measured by bioassay against the current international standard. The yield of growth hormone/gland was approximately 5 mg.

引用

页码：77 / 86

页数：10

共 19 条

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